Competitive PCR–ELISA combines competitive PCR with an ELISA to allow quantitative detection of PCR products. It is based on the inclusion of an internal standard competitor molecule that is designed ...

As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a primer-extension fluorescent-labeling reaction with either P2a or P2b ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

The evolution of PCR technology is shaping the future of diagnostics, especially in viral detection and RNA analysis. In the second episode of our New Frontiers in PCR Technology Series, we have ...

SalivaDirect, an open-source protocol, avoids many of the supply bottlenecks of other tools and could be offered for as little as $10 a test. Long-term GLP-1 exposure drives phosphorylation of a key ...

This guide provides a comprehensive overview of PCR technologies, highlighting the benefits, workflows and applications of endpoint PCR, qPCR and dPCR. Using gene expression profiling as a central ...

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...

Quantitative polymerase chain reaction, or "qPCR", is considered the “second generation” of PCR and was first described in 1992. The past three decades have seen significant advances in qPCR ...

There are many different technologies available to conduct multiplex PCR. However, each comes with its own sets of pros and cons. This infographic compares three pillars of PCR, quantitative PCR, ...

In the nearly two decades since its discovery, the polymerase chain reaction (PCR) and its derivative techniques have revolutionized molecular biology. The exponential nature of the technique allows ...